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Finally, we conduct trajectory analysis using a range of software originally designed by Dr. Graham Dunn and Dr. Daniel Zicha at the Randall Centre, Kings College London. This program returns a Rayleigh test for unimodal clustering of directions where the null hypothesis is uniform distribution of cell trajectories. If the null hypothesis is rejected by the Rayleigh test the cells are showing a significant directional response to CSF-1. This software is not commercially available, however Kinetic Imaging has some software analysis to use with the DCC incorporated into the AQM software.

And Brown, A. F. (1991) A new direct-viewing chemotaxis chamber. J. Cell Sci. 99, 769–775. 8. 8 Jones, G. , Dunn, G. , and Thrasher, A. (2002) Restoration of podosomes and chemotaxis in Wiskott-Aldrich syndrome macrophages following induced expression of WASp. Int. J. Biochem. Cell Biol. 34, 806–815. 9. 9 Orr, A. , Elzie, C. , Kucik, D. , and Murphy-Ullrich, J. E. (2003) Thrombospondin signaling through the calreticulin/LDL receptor-related protein co-complex stimulates random and directed cell migration.

Quickly but carefully invert the cover slip over the two wells in the center of the chamber. It is important that inverting the cover slip over the chamber does not incorporate any bubbles into the chamber as these can affect the gradient (see Note 2). 36 Wells and Ridley Fig. 2. Part-assembled chamber. Once the cover slip is in place, the chamber is sealed on three sides with wax. The fourth side remains unsealed and the medium is removed from the outer well and replaced with chemoattractant using this outer well gap.

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A study in phrasing by Glise, anthony

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